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胶原酶消化分离兔关节软骨细胞及体外聚集培养的观察

Keywords: 软骨细胞,胶原酶,聚集培养,关节软骨,

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Abstract:

目的通过观察采用单一胶原酶消化分离、体外聚集培养获得的兔关节软骨细胞的生物学性状,证实这种获得方法的可靠性。方法取活体成年兔关节软骨,体外采用Ⅱ型胶原酶一次性消化,高密度聚集培养后用倒置显微镜观察细胞形态及生长情况,常规染色及Ⅱ型胶原免疫组化染色法观察软骨细胞表型变化。结果(1)收集细胞总量为(1.2~2.6)×105个?g-1。台盼兰检测细胞活性率平均为96.7%。(2)前三代细胞生长迅速,形态呈短梭形、三角形、多边形等,融合时呈卵圆形。(3)经过聚集扩增培养后Mallory及甲苯胺兰异染反应均为阳性,Ⅱ型胶原免疫组化结果为阳性。结论单一胶原酶消化分离、体外聚集培养这一方法可以获取大量优良的软骨细胞。

References

[1]  Green WT.Behavior of articular chondrocytes in cell culture[J].Clinorthop,1971,(75):248-260.
[2]  Klagsbrun M.Large-scale preparation of chondrocytes[J].Methods Enzymol,1979,58:560-564.
[3]  Shimomura Y,Yoneda T,Suzuki F.Osteogenesis by chondrocytes from growth cartilage of rat rib[J].Caleif Tissue Res,1975,19:179-187.
[4]  Vacanti CA,Cao YL.Neo-cartilage generated from chondrocytes isolated from 100 year old human cartilage [J].Transplant Proc,1994,26(4):1069-1077.
[5]  Brittberg M,Peterson L,Sjiogren-jansson E,et al Articllar cartilage engineering with autologous chondrocyte transplantation.A review of recent developments[J].J Bone Joint Surg Am,2003,85:109-115.
[6]  Chan CK,Anastassiades TP.Anionie glycoconjugates from differentiated and dedifferentiated cultures of bovine articular chondracytes:modulation by TGF-betal[J].In Vitro Cell Dev Biol Anim,1998,34(6):497-498.
[7]  Giannoni P,Canccdda R..Articular chondrocyte culturing for cell-based cartilage repair:needs and perspectives[J].Cells Tissues Organs,2006,184(1):1-15.
[8]  Watt FM.Effect of seeding density on stability of the differentiated phenotype of pig articular chondrocytes in culture[J].J Cell Sci,1988,89:373-378.
[9]  Van Osch GJVM,Vander Veen SW,Verwoerd-Verhoef HL.In vitro redifferentiation of culture-espanded rabbit and human auricular chondrocytes for cartilage reconstruction[J].Plast Reconstr Surg,2001,107:433-440.
[10]  Kamil S H,Kojima K,Vacanti M P,et al.Tissue engineered cartilage:Utilization of autologous serum-free media for condrocyte culture[J].Int J Pediatr Otorhinolaryngol,2007,71(1):71-75.
[11]  Robinson D,Ash H,Nevo Z,et al.Characteristics of cartilage biopsies used for autologous chondrocytes transplantation[J].Cell Transplant,2001,10:203-208.
[12]  余方圆,卢世璧,崔雪梅,等.兔关节软骨细胞聚集培养的生物学观察[J].中华外科杂志,2006,44(12):848-851.

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