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Obestatin抑制胰岛β细胞株INS-1细胞L型钙通道电流

, PP. 849-852

Keywords: 肥胖抑制素,胰岛β细胞株INS-1细胞,电压依赖性L型钙通道,膜片钳

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Abstract:

目的观察obestatin对胰岛β细胞株INS-1细胞L型钙通道电流的影响。方法应用穿孔全细胞膜片钳技术研究obestatin对培养的胰岛β细胞株INS-1细胞膜L型电压敏感钙通道电流(L-typecalciumionchannelcurrent,ICa,L)的影响。结果胰岛β细胞株INS-1细胞钳制膜电位为-20mV时,分别给予胰岛β细胞株INS-1细胞0nM(对照组)和1、10、100nMobestatin灌流5min后,ICa,L峰值呈浓度依赖性降低,各组为给药前的(97±8)%、(91±7)%、(60±9)%和(45±6)%。其中,10nM和100nMobestatin灌流5min后ICa,L峰值与对照组相比显著减小(P<0.01,n=5)。胰岛β细胞株INS-1细胞钳制膜电位分别为-30、-20、-10和0mV时,给予10nMobestatin之前,其ICa,L峰值分别为(-5.48±0.69)、(-5.70±0.68)、(-5.58±0.61)和(-3.90±0.44)pA/pF,灌流10nMobestatin5min后,ICa,L峰值分别下降至(-3.11±0.68)、(-3.60±0.99)、(-3.26±1.03)和(-2.28±0.71)pA/pF,均明显低于obestatin给药前(P<0.01,n=5)。结论obestatin可抑制胰岛β细胞株INS-1细胞L型钙通道电流。

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