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β-catenin和TCF-4在人不同退变程度软骨组织中的表达及意义

Keywords: β-catenin,TCF-4,退变,骨关节炎

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Abstract:

目的通过比较正常人与原发性骨关节炎患者的关节软骨组织中β-catenin和TCF-4的表达,探讨其在骨关节炎发病过程中的作用。方法取因膝骨关节炎行全膝关节置换术的40名患者及因创伤行截肢的10名患者所捐献的关节软骨,行番红O-固绿染色,按改良Mankin评分标准分组。用免疫组化法检测β-catenin的表达;用WesternBlot实验方法检测各组β-catenin、TCF-4的表达。结果免疫组化染色发现β-catenin在无退变组表达为阴性,在轻中度与重度退变组均有表达,以细胞核表达为主,且重度退变组表达明显高于轻中度组(P<0.05)。WesternBlot结果显示β-catenin、TCF-4在退变组软骨组织中表达量均高于无退变组,且重度组表达高于轻中度组(P<0.05)。结论退行性骨关节病软骨组织中β-catenin、TCF-4的表达随软骨组织损伤程度而增加。

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