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钙蛋白酶对控制通气大鼠膈肌组织学的影响

Keywords: 机械通气,膈肌,钙蛋白酶,肌损伤

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Abstract:

目的观察钙蛋白酶对控制通气膈肌组织形态学的影响,以探讨机械通气导致的膈肌功能异常可能的机制。方法SD大鼠18只随机分为对照组、24h控制通气组(CMV组)和钙蛋白酶抑制剂组(calpeptin,CMVC组),观察各组大鼠膈肌组织学和超微结构的变化。结果(1)光镜下显示CMVC组膈肌纤维横截面形状规则,毛细血管内皮略有肿胀,与CMV组相比,未见炎性细胞浸润和中央核的改变;CMVC组膈肌纤维异常组织和结缔组织显著低于CMV组(P<0.05)。(2)电镜下,与CMV组相比,CMVC组膈肌肌原纤维排列整齐,Z线清晰,未见线粒体空泡变;CMVC组膈肌纤维损伤密度显著低于CMV组(P<0.05)。结论钙蛋白酶抑制剂能够明显逆转控制通气所致的膈肌损伤,提示钙蛋白酶可促进通气所致膈肌损伤。

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