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血管内皮生长因子基因多态性与复发性自然流产的关联性研究

Keywords: 血管内皮生长因子,复发性流产,基因多态性

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Abstract:

目的探讨血管内皮生长因子(vascularendothelialgrowthfactor,VEGF)基因-2578C/A,-1154G/A,-460T/C和+936C/T4个多态性位点与复发性自然流产(recurrentspontaneousabortion,RSA)的关联性。方法分别采用聚合酶链式反应-限制性片段长度多态性(PCR-RFLP)法和等位基因特异性扩增-聚合酶链反应法(AS-PCR),检测203名患者和190名健康妇女-2578C/A、-1154G/A、-460T/C和+936C/T多态位点基因型及等位基因频率的分布情况。结果-2578C/A和-1154G/A多态位点的基因型频率、等位基因频率在病例组和对照组间差异均无统计学意义(P>0.05)。-460T/C和+936C/T位点基因型频率等位基因频率,病例组和对照组相比均有统计学差异(P<0.01);VEGF-2578C/A、-460T/C、+936C/T多态位点的单倍型频率在病例组和对照组差异有统计学意义(P<0.01)。结论携带VEGF-460TT基因型及+936T等位基因的基因型可能会增加女性复发性自然流产的发病风险,单倍型VEGF-2578/-460/+936CTT、ATT、CCC和ACC与复发性自然流产的发病可能相关。

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