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卵泡刺激素对上皮性卵巢癌细胞株E-粘连蛋白的表达及侵袭力的影响

Keywords: 上皮性卵巢癌,细胞株,卵泡刺激素,E-粘连蛋白,侵袭力

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Abstract:

目的观察卵泡刺激素(FSH)刺激卵巢癌细胞株中E-粘连蛋白(E-catenin)的表达,研究FSH对卵巢癌细胞株侵袭力的影响。方法20、40、60和80mIU?mL-1浓度FSH作用HO8910、HO8910PM、SKOV3细胞株,分别与0浓度FSH比较,通过Transwell实验测细胞株侵袭力,免疫组化、RealtimePCR检测细胞株Ecatenin蛋白及其mRNA表达情况。结果FSH受体阳性的HO8910、HO8910PM细胞株的侵袭力,在FSH浓度为40、60mIU?mL-1时显著增强(P0.05),其E-catenin蛋白及其mRNA的表达差异无统计学意义(P>0.05)。结论FSH增强卵巢癌细胞株HO8910PM、HO8910的侵袭力,可能与其降低卵巢癌细胞株E-catenin的表达有关。

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