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β-巯基乙醇对胎儿卵巢组织体外培养的影响

DOI: 10.3969/j.issn.1674-6309.2005.01.002, PP. 5-7

Keywords: β-巯基乙醇,胎儿卵巢,组织培养

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Abstract:

目的探讨低分子量的硫醇类化合物-β-巯基乙醇(β-ME)对胎儿卵巢组织体外培养效果的影响。方法取中期引产的20~22周死亡女胎的卵巢,分别在含β-ME50μM、100μMβ-ME(实验组)和不含β-ME(对照组)的培养液内培养3~9d,观察培养后各组卵巢卵泡的形态学变化。结果胎儿卵巢组织经体外培养后可进一步发育,培养后各期卵泡构成比实验组与未培养组、对照组比较差异均有统计学意义(P0.05)。〖HT5”XK〗结论在培养液中添加β-巯基乙醇后,可以更好地维持卵泡的形态,促进卵泡发育。

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