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TLR9在胃癌细胞上的表达及其作用的实验研究

, PP. 770-772

Keywords: CpGODN,MTT,胃癌细胞,TLR9

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Abstract:

目的探讨TLR9在胃癌细胞MGC803上的表达及其激动剂对MGC803细胞增殖和凋亡的影响。方法用RT-PCR方法检测MGC803细胞上TLR9的表达;用TLR9的激动剂CpG寡脱氧核苷酸(CpGoligodexynucleotides,CpG-ODN)10μg?mL-1分别作用MGC803细胞24、48h和72h后,用MTT法检测细胞生长抑制率;流式细胞术观察CpGODN作用MGC803细胞24h后的细胞凋亡现象。结果MGC803细胞上有TLR9的表达;TLR9激动剂CpGODN作用于MGC803细胞不同时间点,均能明显抑制MGC803细胞生长(P<0.01),以早期凋亡为主。结论TLR9激动剂CpGODN对胃癌细胞的生长具有抑制作用。

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