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抗菌肽CecropinB对鸡肠道正常微生物群的影响

, PP. 762-766

Keywords: CecropinB,雏鸡,肠道菌群,DGGE

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Abstract:

目的探讨重组抗菌肽CecropinB对鸡肠道正常微生物群的影响。方法50羽健康雏鸡随机分成5组设盐酸环丙沙星对照组,基因工程抗菌肽cecropinB高、中、低三个剂量组及空白对照组,每组10羽,均以饮水混饮的方式饲喂,分别于42、49d取盲肠、回肠内容物进行聚合酶链式反应-变性梯度凝胶电泳(PCR-DGGE),对肠道的几种优势菌的多样性进行研究,并对回肠16SrDNA进行全序列克隆、测序。结果雏鸡盲肠菌群在42日龄和49日龄之间相似性达到50%。相同日龄各组间盲肠菌群分为两大类,其中抗菌肽不同剂量组间相似性为73%及以上,而抗菌肽不同剂量组与空白对照组及抗生素对照组之间的相似性分别为61%以上和50%。各组雏鸡回肠显示两大类优势菌群,抗生素对照组为一大类,其他组别为一大类,两者间的相似性为59%;各组内样品之间相似性在92%以上,组间相似性在70%左右。对回肠内容物16SrDNA克隆、测序显示,抗菌肽高剂量组比空白对照组多出一条与厌气弯杆菌同源性为78%的条带。结论CecropinB作为一种饲料添加剂对42与49日龄雏鸡肠道菌群无明显影响。

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