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宁夏绵羊脑组织博尔纳病病毒的自然感染状况

, PP. 755-758

Keywords: 博尔纳病病毒BDVp24基因,BDVp40基因,荧光定量巢式逆转录PCR,绵羊脑组织

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Abstract:

目的了解宁夏地区绵羊脑组织博尔纳病病毒(Bornadiseasevirus,BDV)的自然感染情况,并分析其与德国马源性标准病毒株He/80的同源性。方法应用荧光定量巢式逆转录PCR(FQ-nRT-PCR)和RT-PCR分别检测宁夏地区163例绵羊脑组织中BDVp24、BDVp40基因片段,并对BDVp24阳性扩增产物进行测序,与国外标准病毒株进行比较。结果163例绵羊脑组织中6例BDVp24、BDVp40均阳性,阳性率为3.68%(6/163)。测序后经基因序列比对,本实验检测阳性BDVp24片段的核苷酸序列与He/80同源性为100%。结论宁夏地区绵羊脑组织中可能存在动物源性BDV的隐性感染,阳性BDVp24核苷酸序列与He/80具有高度同源性。

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