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CyclinE蛋白在食管鳞状细胞癌组织中的表达及意义

, PP. 674-676

Keywords: 食管癌,CyclinE,免疫组化,定量分析

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Abstract:

目的观察CyclinE在食管鳞状细胞癌和癌周正常食管组织的表达,从量化角度阐明其在食管鳞状细胞癌组织中表达的病理学意义。方法常规石蜡包埋、HE切片确诊,免疫组化MaxVision法检测CyclinE在食管鳞状细胞癌和癌周正常食管组织的定位和表达,Image-proplus图像分析软件对其表达强度进行定量分析,并用表达的阳性单位(positiveunit,PU)反映其表达强度。结果CyclinE蛋白在食管鳞状细胞癌和癌周正常食管组织中的表达主要定位在细胞核。食管鳞状细胞癌表达的PU值高于癌周正常食管组织(P0.05)。结论CyclinE蛋白表达程度与食管鳞状细胞癌及分化程度具有相关性,可能成为判断其指标之一。

References

[1]  Koff A,Cross F,Fisher A,et al. Human CyclinE,a new cyclin that interacts with two members of the CDC2 gene family[J].Cell,1991,66:1197-1206.
[2]  Zhang H. Life without kinase: cyclin E promotes DNA replication licensing and beyond[J]. Mol Cell, 2007, 25(2):175-176.
[3]  Bondi J,Husdal A,Bukholm G,et al. Expression and Gene Amplification of Primary(A,B1,D1,D3 and E)and Secondary(C and H)Cyclins in Colon Adenocarcinomas and Correlation with Patient Outcome[J]. J Clin Pathol,2005,58(5):509-514.
[4]  Elise Bales,Lisa Mills,Nancy Milam,et al. The low molecular weight cyclinE isoforms augment angiogenesis and metastasis of human melanoma cells in vivo[J]. Cancer Res, 2005, 65(2):692-697.
[5]  Rosen DG,Yanq G,Deavers MT,et al. CyclinE expression is correlated with tumor progression and predicts a poor prognosis in patients with ovarian carcinom [J]. Cancer,2006,106(9):1925-1932.
[6]  金春亭,张林西,李海军,等.人食管鳞癌中细胞周期调节基因蛋白的表达[J].世界华人消化杂志,2005,13(15):1909-1912.
[7]  Matsumoto M,Furihata M,Ishikawa T, et al.Comparison of deregulated expression of cyclinD1 and cyclinE with that of cyclin-dependent kinase 4(CDK4)and CDK2 in human oesophageal squamous cell carcinoma[J].Br J Cancer,1999,80:256-261.
[8]  蔡扬,何园,卢虹,等. 口腔鳞状细胞癌cyclin E 及p21waf1 蛋白定量检测及意义[J]. 中国肿瘤临床,2008,35(3):142-144.
[9]  张晓丽,程建君,蒋丽娜,等. P16和CyclinE蛋白在食管鳞状细胞癌中的表达及意义[J]. 现代肿瘤医学,2005,13(3):340-342.
[10]  Anayama T,Furihata M,Ishikawa T,et al.Positive correlation between p27Kip1 expression and progression of human esophageal squamous cell carcinoma[J].Int J Cancer,1998,79:439-443.

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