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结核分枝杆菌phoS2基因的克隆及序列分析

, PP. 669-673

Keywords: 结核分枝杆菌,phoS2基因,克隆,序列分析

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Abstract:

目的扩增结核分枝杆菌phoS2基因,并将其克隆至质粒pGEMT中进行核苷酸序列特性分析。方法以结核菌标准菌株H37Rv为模板,通过PCR技术扩增出结核分枝杆菌phoS2抗原基因,将其重组到pGEM-T载体后进行酶切鉴定及序列测定和生物信息学分析。结果成功扩增出结核分枝杆菌phoS2抗原基因,测序表明该片段开放阅读框为927bp,与已发表基因核苷酸序列相比,同源性为81%,推导编码氨基酸序列同源性为87%。结论成功克隆phoS2基因,为结核病原核表达及相关研究奠定了基础。

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