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槐定碱预处理对LPS诱导的RAW264.7 巨噬细胞c-Jun表达的影响

, PP. 161-164

Keywords: 槐定碱,LPS,RAW264.7巨噬细胞株,c-Jun

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Abstract:

目的观察槐定碱预处理对LPS刺激RAW264.7巨噬细胞c-Jun表达的影响,探讨槐定碱抗内毒素机制。方法培养RAW264.7巨噬细胞,以槐定碱(31.25mg?L-1)预处理细胞24h后给予LPS(E.coliO55B5)100μg?L-1刺激细胞,并于刺激后5、30、60、120min收集细胞;再以槐定碱31.25、15.63、7.81mg?L-1三个浓度同上预处理细胞,于LPS刺激后60min收集细胞。利用免疫细胞化学技术检测RAW264.7巨噬细胞中c-Jun的表达。结果单独槐定碱对c-Jun表达无影响;LPS模型组各时间点c-Jun阳性细胞数均显著高于巨噬细胞对照组(P<0.01),且随LPS刺激时间延长而升高,持续升至120min;槐定碱预处理组在LPS作用后30、60、120min时c-Jun阳性细胞数均较同时间点LPS模型组降低,差异有统计学意义(均P<0.01),但槐定碱预处理组各时间点c-Jun阳性细胞数无明显变化。不同浓度槐定碱(31.25、15.63、7.81mg?L-1)预处理细胞,对LPS刺激的c-Jun表达均有显著抑制作用(均P<0.01),且31.25mg?L-1的作用强于另两个浓度(15.63、7.81mg?L-1),差异有统计学意义(均P<0.05)。结论槐定碱预处理RAW264.7巨噬细胞能显著抑制LPS诱导的c-Jun蛋白表达,其作用有剂量依赖性。

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