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HCV感染后小鼠小胶质细胞瘤细胞炎性因子分泌特点的研究

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Keywords: BV2细胞,丙型肝炎病毒,肿瘤坏死因子-α,白介素-12,白介素-6

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Abstract:

目的了解丙型肝炎病毒(HCV)感染小鼠小胶质瘤细胞(BV2)后Th1/Th2类细胞因子的变化,探讨HCV感染后小胶质细胞的免疫分泌效应。方法体外培养BV2细胞,随机分为HCV阳性血清组、正常人血清组、空白对照组。分别作用24h用倒置显微镜观察细胞形态并收集上清液,ELISA法检测上清液中白介素-12(IL-12)、肿瘤坏死因子α(TNF-α)和白介素-6(IL-6)的含量。结果倒置显微镜下观察显示,HCV阳性血清感染后BV2细胞呈圆形,突起变粗短,部分细胞聚集成团。HCV阳性血清感染组TNF-α、IL-12、IL-6含量均高于正常人血清组与空白对照组,差异有统计学意义(P0.05)。结论HCV感染BV2细胞后Th1类细胞因子TNF-α、IL-12、和Th2类细胞因子IL-6分泌增强,可能与HCV感染CNS引起的早期炎症损伤有关。

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