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4种植物激素对黄花蒿叶片中倍半萜积累的影响

, PP. 1039-1045

Keywords: 青蒿素,植物生长调节剂,黄花蒿,domesticoside,倍半萜

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Abstract:

目的研究3种常用植物激素6-BA(6-苄基氨基嘌呤)、GA3(赤霉素)、CCC(矮壮素)及首次从黄花蒿中分离得到的植物生长调节剂Domesticoside(DOM)对黄花蒿叶片中青蒿素(Artemisinin)及相关倍半萜青蒿乙素(ArteannuinB)、3α-hydroxy-1-deoxyartemisinin、青蒿酸(Artemisinicacid)含量积累的影响。方法采用无土栽培实验,分别以6-BA(50、100、200mg?L-1)、GA3(50、400、600mg?L-1)、CCC(300、600、1200mg?L-1)及DOM(0.44、0.87、1.74mg?L-1)连续叶面喷施3周。结果高、中、低浓度CCC干预第1周青蒿素含量分别增至(0.58±0.29)%、(0.40±0.18)%、(0.30±0.11)%,与对照组相比分别提高了14.5、9.0、6.5倍(P<0.05);高、中、低浓度DOM干预第1周青蒿素含量增至(0.41±0.14)%、(0.55±0.21)%、(0.44±0.06)%,与对照组相比分别提高了9.3、12.8、10倍(P<0.05);CCC、DOM对青蒿乙素影响与青蒿素相似,对青蒿酸作用相反,而对3α-hydroxy-1-deoxyartemisinin无影响。结论不同浓度DOM和CCC均能显著提高黄花蒿青蒿素、青蒿乙素含量,可作为黄花蒿人工栽培的植物生长调节剂。

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