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黄体生成素干预对体外培养小鼠卵巢VEGF和Survivin表达的影响

Keywords: 黄体生成素,存活素,血管内皮生长因子,小鼠卵巢,体外培养

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Abstract:

目的探索黄体生成素(LH)对小鼠卵巢体外培养后血管内皮生长因子(VEGF)和存活素(Survivin)表达的影响。方法将从8周龄小鼠新鲜取材的半卵巢分别在无LH(培养对照组)和添加LH(0.15IU?mL-1)干预下培养3、6、12、24h后通过免疫组化法观察半卵巢VEGF、Survivin的表达。结果培养组VEGF、Survivin表达比新鲜对照组降低且呈逐渐降低趋势;在培养3、6、12h阶段,LH0.15干预后VEGF阳性表达高于培养对照组(P<0.05);3h和12hSurvivin的表达均高于培养对照组(P<0.05)。结论LH体外干预可上调小鼠卵巢的VEGF、Survivin表达,VEGF和Survivin表达呈正相关。

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