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LIG4mRNA与XRCC4mRNA在宫颈癌中的表达及临床意义

, PP. 215-218

Keywords: 宫颈癌,DNA连接酶4,X射线修复交叉互补基因4

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Abstract:

目的探讨宫颈癌组织中DNA连接酶4(LIG4)及X射线修复交叉互补基因4(XRCC4)的mRNA表达水平及临床意义。方法应用实时荧光定量RT-PCR技术检测61例宫颈癌和20例正常宫颈组织中LIG4和XRCC4的mRNA表达水平,分析两种基因的表达与宫颈癌分期、分化程度及盆腔淋巴结转移之间的关系。结果LIG4mRNA和XRCC4mRNA在宫颈癌组织及正常宫颈组织中的表达分别为(2.88±1.97)、(5.02±1.87)和(2.16±0.99)、(3.72±1.36),差异有统计学意义,P值分别为0.00和0.00;宫颈癌患者盆腔淋巴结有转移组和无转移组中的表达分别为(0.61±0.17)、(0.41±0.28)和(0.80±0.31)、(0.58±0.26),差异有统计学意义,P值分别为0.00和0.01;宫颈癌低分化组和中高分化组中的表达分别为(1.81±0.99)和(2.03±1.25)、(1.50±0.76)和(1.45±0.57),差异无统计学意义(P>0.05)。LIG4mRNA在Ⅱ期和Ⅲ期宫颈癌组织中的表达分别为(0.71±0.34)和(0.96±0.36),差异有统计学意义,P=0.01。LIG4mRNA与XRCC4mRNA在宫颈癌组织中表达呈正相关(r=0.38,P=0.00)。结论LIG4mRNA和XRCC4mRNA在宫颈癌组织中的异常表达与宫颈癌的发展及盆腔淋巴结转移密切相关。

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