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真核重组质粒pEGFP-FcγRIIb在NIH3T3细胞及 BALB/c小鼠体内的表达

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Keywords: FcγRIIB,pEGFP-FcγRIIb,BALB/c小鼠

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Abstract:

目的观察真核重组质粒pEGFP-FcγRIIb在真核细胞NIH3T3及BALB/c小鼠体内的表达,为后续的体内研究奠定基础。方法采用脂质体法将重组质粒pEGFP-FcγRIIb转染到NIH3T3中,G418筛选3周后,采用Westernblot方法鉴定稳定表达的产物。进一步将重组质粒注射入BALB/c小鼠肌肉中,用Westernblot及免疫组织化学法检测人FcγRIIB的表达情况。结果转染重组质粒pEGFP-FcγRIIb的NIH3T3细胞及注射重组质粒的小鼠肌肉中表达了人的FcγRIIB蛋白,分子量约为37kDa。结论成功获得了真核重组质粒pEGFP-FcγRIIb在NIH3T3及BALB/c小鼠体内的表达,为进一步研究FcγRIIB的功能奠定了基础。

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