全部 标题 作者
关键词 摘要

OALib Journal期刊
ISSN: 2333-9721
费用:99美元

查看量下载量

相关文章

更多...

腺病毒介导的RNA干扰降低VEGF转录抑制血管形成的研究

, PP. 84-87

Keywords: 血管生成,血管内皮生长因子,RNA干扰

Full-Text   Cite this paper   Add to My Lib

Abstract:

用细菌内同源重组的方法构建了针对VEGF的siRNA表达重组腺病毒AdH1-siRNA/VEGF,感染HUVEC细胞,观察该病毒对HUVEC细胞体外增殖和形成微血管的干扰作用.通过Real-timeRT-PCR实验表明,AdH1-siRNA/VEGF可特异性地下调血管内皮细胞(HUVEC)中的VEGFmRNA水平.与对照组相比,VEGF的mRNA水平下降为50%,AdH1-siRNA/VEGF对HUVEC细胞增殖有干扰作用.加入病毒9d后,对照组的细胞平均计数为31.5×104/μL,干扰病毒对照组为28×104/μL,AdH1-siRNA/VEGF干扰组为21.5×104/μL.AdH1-siRNA/VEGF对HUVEC细胞在Matrigel上形成微血管有干扰作用,对照组每HPF形成微血管数量为10.75条,干扰病毒对照组为10.25条,实验组AdH1-siRNA/VEGF每HPF形成微血管数量为7条.证明AdH1-siRNA/VEGF可以干扰血管形成.

References

[1]  [ Fo lkman J. Ang iogenes is in cancer, vascular, rheum a to id and o ther disease[ J]. NatM ed, 1995, 1( 1): 27- 31.
[2]  [ Fe rrara N, H enze lW J. P itu itary fo llicular ce lls secre te a nove l hepar in-b inding grow th factor spec ific for vascular endothe lial ce lls[ J]. B iochem B iophys Res Commun, 1989, 161( 2): 851- 858.
[3]  [ H annon G J. RNA interference[ J]. Nature, 2002, 418( 6894): 244- 251.
[4]  [ Shen C, Buck A K, Liu X, et a .l Gene silenc ing by adenov irus-de livered siRNA[ J]. FEBS Lett, 2003, 539: 111- 114.
[5]  [ Sam brook J, Fritsch E F, M aniatis T. Mo lecu la r C loning[M ]. 2nd ed. Co ld Spr ing H arbor, New Yo rk: Co ld Spring H arbor Labo ra tory Press, 1989.
[6]  [ Annabi B, Thibeault S, Lee Y T, et a.l M atr ix m e tallopro te inase regulation of sph ingosine-1-phosphate- induced ang iogenic properties o f bone m arrow strom al ce lls[ J] . Exp H em ato,l 2003, 31: 640- 649.
[7]  [ Zhang L, Yang N, M oham ed-H adleyA, et a.l Vecto r-based RNA,i a nove l tool fo r iso fom-specific kno ck-down of VEGF and ant-i ang iogenes is g ene therapy of cance r[ J]. B iochem B iophys Res Commun, 2003, 303: 1 169- 1 178.
[8]  [ Re ich S J, Fo sno t J, Kurok iA, et a.l Sm a ll inter fe ring RNA ( siRNA) targetingVEGF effectively inhabits o cu lar neovasculariza tion in a m ousem oda l[ J] . M o lV is, 2003, 9: 210- 216.

Full-Text

Contact Us

service@oalib.com

QQ:3279437679

WhatsApp +8615387084133