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华西医学  2011 

胃癌组织中过氧化酶增殖因子活化受体γ和血管内皮生长因子C的表达及临床意义

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Keywords: 胃癌,氧化酶增殖因子活化受体,血管内皮生长因子C,逆转录-聚合酶链反应

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Abstract:

【】 目的 研究过氧化酶增殖因子活化受体γ(peroxisomeproliferator-activatedreceptorgamma,PPARγ)和血管内皮生长因子C(vascularendothelialgrowthfactorC,VEGF-C)在胃癌组织中表达的相关性,分析其表达与临床病理因素之间的关系。 方法 2005年3月-2009年3月间采用逆转录-聚合酶链反应方法检测36例胃癌手术标本中PPARγ和VEGF-CmRNA的表达,同时选取相同患者的胃正常组织作为对照。 结果 PPARγmRNA在胃癌中的表达量高于癌旁正常组织,两者的差异有统计学意义(P=0.007);VEGF-C在胃癌中的表达量高于癌旁正常组织,两者的差异有统计学意义(P=0.004);PPARγ的表达与VEGF-C表达无关联性(r=0.135,P=0.414);PPARγ表达与胃癌组织中浸润程度有关(χ2=4.620,P=0.032)、淋巴结转移有关(χ2=15.753,P=0.000)和临床病理分期有关(χ2=4.610,P=0.032);VEGF-C表达与胃癌组织中淋巴结转移有关(χ2=4.729,P=0.030)、远处转移有关(χ2=4.064,P=0.044)和临床病理分期有关(χ2=6.300,P=0.012)。 结论 PPARγ和VEGF-C可能在胃癌新生淋巴管形成中起重要作用,两者的表达水平与胃癌患者的病情判断及预后评价密切相关。【Abstract】 Objective Toinvestigatethesignificanceofexpressionsofperoxisomeproliferator-activatedreceptorgamma(PPARγ)andvascularendothelialgrowthfactorC(VEGF-C)ingastriccarcinomaaswellastheircorrelation,andtostudytherelationshipbetweentheexpressionsandclinicopathologiccharacteristicsofgastriccarcinoma. Methods Thirty-sixpairsofnormalmucosaandcancerspecimenswereobtainedfrompatientswhohadundergonegastricoperationforprimarygastriccarcinomaandsubjectedtoreversetranscription-ploymerasechainreaction(RT-PCR)forPPARγandVEGF-CmRNAdetection. Results ThepositiverateandlevelofPPARγmRNAexpressionwerehigheringastriccancertissuesthanthoseinnormalgastricmucosa(P=0.007).ThepositiverateandlevelofVEGF-CmRNAexpressionwerealsohigheringastriccancertissuesthanthoseinnormalgastricmucosa(P=0.004).Simultaneously,theexpressionofPPARγwasnotcorrelatedwiththatofVEGF-C(r=0.135,P=0.414),whilethehighlyproductionsofPPARγandVEGF-Cingastriccarcinomaswerebothassociatedwiththelymphnodemetastasisandtheclinicalstage(P<0.05). Conclusion PPARγandVEGF-Cmayplayanimportantroleinthelymphangio-genesisofgastriccancer,anduniteddetectionofPPARγandVEGF-Cexpressionsmaybecorrelatedwithmakingdiagnosis,evaluatingprognosisinpatientswithgastriccanceratthesametime.

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