幽门螺杆菌NCTC11637株Hp1501基因序列的测定与生物信息学分析

目的　幽门螺杆菌NCTC11637菌株Hp1501基因进行序列测定，并运用生物信息学软件对其进行分析。方法　用聚合酶链反应方法从幽门螺杆菌NCTC11637菌株基因组DNA扩增Hp1501基因，T-A克隆，鉴定后测序，将基因序列向GeneBank提交并申请登录号，用生物信息学软件分析其生物学特性。结果　成功获取幽门螺杆菌NCTC11637株Hp1501基因序列，获得GeneBank登录号JF820815。软件分析表明，序列全长为1164bp，与幽门螺杆菌国际标准株26695和J99的基因序列一致性为96%～97%，氨基酸序列一致性为97%～98%；软件预测其编码的前59个氨基酸为信号肽，其编码的核心肽为幽门螺杆菌外膜蛋白。结论　成功测定幽门螺杆菌NCTC11637菌株Hp1501基因序列并预测出其生物学特性，为进一步研究其功能，阐明其致病机制奠定了基础。Objective　TodeterminethesequenceofHp1501genefromH.pyloriNCTC11637andanalyzethesequencewithbioinformaticssoftware.Methods　Polymerasechainreaction(PCR)wasusedtoamplifytheHp1501genefromchromosomalDNAofH.pyloriNCTC11637.AfterT-Aclone,theamplifiedDNAsequencewasdeterminedandthegenesequencewassenttoGeneBankforanalysiswithbioinformaticssoftware.Results　Hp1501genefromH.pyloriNCTC11637wassuccessfullyattained,andtheloggingnumberforGeneBankwasJF820815.Theanalysisshowedthatthegenesequencewas1164bpinlength,96%-97%identicalinDNAsequenceand97%-98%identicalinaminoacidsequencecomparedwithstandardstrain26695andJ99.Theforward59aminoacidsweresignalpeptideandthecorepeptidewasoutermembraneproteinofH.pyloribasedonthesoftwareprediction.Conclusions　ThesequenceandbionomicsofHp1501genefromH.pyloriNCTC11637hasbeendeterminedsuccessfully.ItprovidesasolidbaseforthefurtherresearchofthebiologicalfunctionandpathogenicitymechanismofH.pylori.