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华西医学  2013 

355 nm和407 nm激光激发Hoechst 33342检测细胞凋亡的比较研究

DOI: 10.7507/1002-0179.20130274, PP. 875-878

Keywords: 355nm激光,407nm激光,流式细胞仪,凋亡,Hoechst33342

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Abstract:

目的 比较使用流式细胞仪355nm和407nm激光器激发Hochest33342检测细胞凋亡。方法 通过ATO药物诱导急性早幼粒白血病细胞(NB4)及血清饥饿法诱导人肺癌细胞(NCl-H292)细胞凋亡,取24、48h时间点收集细胞,进行Hoechst33342-碘化丙啶(PI)双染,分别在配置有两种激光器的流式细胞仪上检测细胞凋亡。结果 细胞经处理后24h,355nm激光器检测NB4细胞凋亡率Hoechst33342+/PI-(28.20±4.80)%;NCl-H292细胞凋亡率Hoechst33342+/PI-(22.47±2.78)%。407nm激光器检测NB4细胞凋亡率Hoechst33342+/PI-(25.10±6.19)%。NCl-H292细胞凋亡率Hoechst33342+/PI-20.47±1.46%。处理后48h,355nm激光器检测NB4细胞凋亡率Hoechst33342+/PI-(33.60±3.75)%。NCl-H292细胞凋亡率Hoechst33342+/PI-(26.77±1.16)%。407nm激光器检测NB4细胞凋亡率Hoechst33342+/PI-(29.47±2.33)%。NCl-H292细胞凋亡率Hoechst33342+/PI-(31.47±3.05)%。两种细胞处理后比处理前凋亡率明显升高,但355nm激光器与407nm激光器检测的凋亡结果差异不明显(P>0.05)。结论 407nm激光器激发Hoechst33342可检测细胞凋亡。

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