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华西医学  2014 

慢性阻塞性肺疾病合并2型糖尿病大鼠模型的建立

DOI: 10.7507/1002-0179.20140610, PP. 2012-2015

Keywords: 吸烟,慢性阻塞性肺疾病,糖尿病,大鼠

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Abstract:

目的 探讨制作慢性阻塞性肺疾病(COPD)合并2型糖尿病大鼠模型的方法。方法 将80只Sprague-Dawley(SD)雄性大鼠随机分成COPD组、糖尿病组、COPD+糖尿病组和正常组,每组20只。采用单纯熏香烟法制备COPD大鼠动物模型,利用链脲佐菌素诱导SD大鼠产生糖尿病,两种方法协同产生COPD+糖尿病的大鼠模型,正常组常规饲养。3个月后作常规病理检查,血糖测定。结果 COPD+糖尿病组大鼠肺组织病理学改变支气管黏膜上皮脱落严重,肺间质及气道管壁有大量的炎性细胞浸润,肺泡结构破坏,部分融合成肺大疱。造模成功后COPD+糖尿病组的血糖水平为(27.1±1.1)mmol/L,与其他3组比较差异有统计学意义(P<0.05)。结论 被动吸烟方式能够建立COPD大鼠模型,利用链脲佐菌素诱导SD大鼠产生糖尿病,两种方法协同产生COPD合并糖尿病的大鼠模型,为进一步医学研究提供动物模型。

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