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华西医学  2013 

新型玻璃化冷冻法对人卵巢组织超微结构保存的研究

DOI: 10.7507/1002-0179.20130180, PP. 567-570

Keywords: 玻璃化冷冻,人卵巢组织,超微结构,间质细胞

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Abstract:

目的 探讨新型玻璃化冷冻法对人卵巢组织超微结构的保存效果,尤其是对卵巢间质细胞的保存效果。方法 收集2007年6月-2009年1月在我院行妇科手术的患者卵巢组织共8例,使用新型玻璃化冷冻法和慢速冷冻法保存,比较两种冷冻方法对于始基卵泡卵母细胞、颗粒细胞、卵泡周围间质细胞超微结构保存效果。结果 对于始基卵泡卵母细胞和颗粒细胞,发现两种冷冻方法之间无显著差异(P>0.05);在间质细胞保存中,新型玻璃化冷冻法与慢速冷冻法相比较,正常间质细胞百分率增加(P<0.05)。结论 新型玻璃化冷冻法与慢速冷冻法相比能更好地保存卵泡周围间质细胞,在深低温保存人卵巢组织中具有较广阔的应用前景。

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