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华西医学  2014 

罗格列酮对老年糖尿病大鼠认知障碍改善的作用机制研究

DOI: 10.7507/1002-0179.20140504, PP. 1642-1646

Keywords: 老年糖尿病,罗格列酮,认知障碍,C反应蛋白,核转录因子-κB

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Abstract:

目的 观察罗格列酮对老年糖尿病大鼠认知功能、血清高敏C-反应蛋白(hs-CRP)水平及海马组织核转录因子-κB(NF-κB)、白细胞介素-6(IL-6)、肿瘤坏死因子-α(TNF-α)表达的作用。方法 将30只20~22月龄老年Wistar大鼠随机分为3组对照组(n=6)予生理盐水腹腔注射;糖尿病组(n=12)和罗格列酮组链脲佐菌素55mg/kg腹腔注射;造模后罗格列酮组以罗格列酮4mg/(kg?d)+无菌水混悬液灌胃,糖尿病组和对照组以等量无菌水灌胃,共8周。8周后应用Morris水迷宫实验评价各组大鼠的认知功能。检测各组大鼠血清hs-CRP水平、海马区域NF-κB、IL-6、TNF-α的表达。结果 罗格列酮组、糖尿病组Morris水迷宫测试中逃避潜伏期分别较正常对照组延长(P<0.05);与糖尿病组相比较,罗格列酮组Morris水迷宫测试中潜伏期缩短(P<0.05),中心区停留时间百分比和通过原平台位置次数增加(P<0.05)。与对照组相比,罗格列酮组和糖尿病组血清hs-CRP水平,NF-κB、IL-6、TNF-α表达升高(P<0.01);罗格列酮组血清hs-CRP水平、NF-κB、IL-6、TNF-α表达较糖尿病组相比降低(P<0.05)。结论 糖尿病大鼠认知功能受损与血清CRP水平相关。罗格列酮对糖尿病大鼠的认知功能减退有改善作用,其机制可能与降低血清CRP水平,抑制海马组织NF-κB信号,降低IL-6、TNF-α的表达有关。

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