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华西医学  2014 

胃癌中钙黏附分子启动子甲基化与幽门螺杆菌感染及肿瘤生物学特性的关系

DOI: 10.7507/1002-0179.20140012, PP. 43-46

Keywords: DNA甲基化,钙黏附分子启动子,E-钙黏蛋白,甲基化特异性聚合酶链式反应,幽门螺杆菌,胃癌

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Abstract:

目的 建立甲基化特异性聚合酶链式反应方法检测组织中钙黏附分子(CDH1)的甲基化程度;探索CDH1启动子的甲基化与幽门螺杆菌(HP)感染及与肿瘤分化、浸润、淋巴及远处转移之间的关系。方法 采用热转化甲基化特异性聚合酶链式反应法检测2008年1月-2009年12月共40例胃癌手术标本中CDH1的启动子甲基化情况,并回顾性地分析CDH1的启动子甲基化与HP感染、肿瘤分化、浸润、淋巴及远处转移等肿瘤生物学特性的统计学关联。结果 胃癌组CDH1基因甲基化阳性率高于对照组(67.5%、12.5%,P<0.05)。胃癌组中低-未分化肿瘤组CDH1基因甲基化阳性率高于高-中分化肿瘤组(80.6%、22.2%,P<0.05);胃癌组中HP阳性患者的CDH1基因甲基化率高于HP阴性患者(78.1%、25.0%,P<0.05);而胃癌组中CDH1基因甲基化阳性率与患者性别、肿瘤浸润程度、淋巴转移及远处转移无明显关系(P>0.05)。结论 CDH1基因甲基化可能参与了胃癌的发展过程,并且CDH1基因甲基化可能导致了肿瘤分化程度的降低。CDH1基因甲基化与胃癌患者的HP感染之间可能存在密切关系,提示HP感染可能参与了抑癌基因甲基化失活及肿瘤演变的发展过程。

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