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华西医学  2010 

骨形成蛋白-2对室管膜前下区神经干细胞DLX5表达的影响

, PP. 2177-2180

Keywords: 神经干细胞,室管膜前下区,DLX5,骨形成蛋白-2,Noggin

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Abstract:

【】 目的 探讨骨形成蛋白-2(bonemorphogeneticprotein-2,BMP-2)对室管膜前下区(anteriorsubventricularzone,SVZa)神经干细胞DLX5表达的影响。 方法 体外培养SVZa神经干细胞,用BMP-2及其拮抗剂Noggin诱导SVZa神经干细胞,分别用免疫荧光染色和逆转录-聚合酶链反应(RT-PCR)检测DLX5表达变化。 结果 BMP-2组SVZa神经干细胞DLX5蛋白表达和DLX5mRNA表达水平明显高于对照组(P<0.05),且该效应能被其拮抗剂Noggin特异性地抑制。 结论 BMP-2是DLX5上游调节基因,可促进SVZa神经干细胞DLX5的表达。?【Abstract】 Objective Toinvestigatetheeffectofbonemorphogeneticprotein-2(BMP-2)onexpressionofDLX5ofneuralstemcellsinanteriorsubventricularzone(SVZa). Methods TheneuralstemcellsofSVZawereseparatedandculturedinvitro,whichwereinducedbyBMP-2andNoggin.ImmunofluorescencestainingandRT-PCRwereemployedtoassaytheexpressionofDLX5. Results ThepercentagesofexpressionofDLX5proteinandDLX5mRNAinBMP-2groupweremuchhigherthanthoseinthecontrolgroup(P<0.05).AndthisinductioncouldbespecificallyblockedbyNoggin. Conclusion BMP-2isanupstreamgeneofDLX5;BMP-2canpromotetheexpressionofDLX5oftheneuralstemcellsofSVZa.

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