机采血小板实验室保存期间的生化改变

【】目的探讨机采血小板在实验室内保存期间的生化改变及其意义。方法采用ROCHEcobasb221和OLYMPUSAU400测定血小板样本的pH值、PO2、PCO2、葡萄糖（GLU）浓度、乳酸脱氢酶（LDH）释放量。结果保存期间pH值由7.311（第1天）降至7.116（第5天）；PO2波动于106.7～119.5mmHg（1mmHg=0.133kPa）；PCO2波动于25.0～35.8mmHg；GLU浓度呈进行性下降，由18.8mmol/L降至15.2mmol/L；LDH浓度显著增加，由150mmol/L（第1天）增至259mmol/L（第5天）。结论血小板在保存期内，其生化参数会随着保存时间发生改变。而这些参数作为间接指标对机采血小板是否被细菌污染具有一定的指导意义。？【Abstract】ObjectiveToobservethebiochemistrychangesofapheresisplatelets(AP),includingpH,PO2,PCO2,GLU,LDH,withinthestoragetimeanditssignificance.MethodsThepH,PO2,PCO2,GLUandLDHofplateletsamplesweredetectedbyROCHEcobasb221andOLYMPUSAU400.ResultsDuringthestoragetime,thepHvalueofplateletdecreasedgraduallyfrom7.311(thefirstday)to7.116(thefifthday);thePO2fluctuatedfrom106.7mmHg(1mmHg=0.133kPa)to119.5mmHg;thePCO2fluctuatedfrom25.0mmHgto35.8mmHg;theconcentrationofGLUdecreasedfrom18.8mmol/Lto15.2mmol/L;theconcentrationofLDHincreasedobservablyfrom150mmol/L(thefirstday)to259mmol/L(thefifthday).ConclusionTherewereobservablychangesofbiochemistryoftheAPduringstoragetime,andthesechangesmaybetheindicatorsofplateletscontamination.