一种兔耳中期增生性瘢痕动物模型的建立

【】目的建立兔耳中期瘢痕动物模型，寻找兔耳瘢痕形成的最佳位点。方法选用日本大耳白兔20只，在兔耳腹侧选定6个位点，作直径1cm直达软骨表面的皮肤全层及软组织缺损240个。创面暴露，于伤后7d去除软骨上面的肉芽及血浆痂壳一次。术后连续3个月观察创面自然愈合及瘢痕增生情况;用HE及苦味酸-天狼星红染色观察瘢痕形成及胶原分布情况;用计算机图像分析系统测定胶原含量。结果兔耳腹侧可制作类似人的增生性瘢痕模型，瘢痕的发生率42.5%~56.7%，瘢痕增生的高峰在造创后30~50d。不同位点瘢痕增生程度不同，胶原含量也不同。结论兔耳腹侧可建立中期瘢痕动物模型，兔耳腹侧的中分和耳尖外侧部分是制作兔耳增生性瘢痕的理想位点。【Abstract】ObjectiveToestablishananimalmodelofintermediatestagehypertrophicscarontherabbitearsandtofindoutthebestsitesofscarformation.MethodsAtotalof240full-thicknessskinandtissuedefectdirectingaccesstothecartilagesurfacewascreatedontheventralsidein20Japanwhiterabbitsandeachearcontain6defectsites.Thewoundwastreatedbyexposuremethod.Onthe7thdayafteroperation,thegranulationtissueandplasmashellwereremovedonthecartilage.Woundhealingandscarproliferationundernaturalconditionwereobservedcontinuouslyfor3months.ThescarformationandcollagendistributionwereobservedbyHEandSiriusredstaining,andthecollagencontentwasanalyzedbyusingcomputerimageanalysissystem.ResultsTheventralwoundofrabbit’searsproducedhypertrophicscarsimilartohumanhypertrophicscar,theincidenceofscarwasbetween42.5%to56.7%.Thepeakofscarproliferationwasin30daysto50daysafteroperation.Thedegreeofscarproliferationandcollagencontentvariedatdifferentsites.ConclusionTheventralwoundofrabbit’searscanproduceintermediatestagehypertrophicscarmodel,themiddlesitesandthelateraleartipareidealsiteformaddinganimalmodelofhypertrophicscarontherabbitears.？