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华西医学  2011 

肥大细胞膜稳定剂对大鼠急性结肠炎的影响

, PP. 1142-1145

Keywords: 肥大细胞,色甘酸二钠,葡聚糖硫酸钠,溃疡性结肠炎,大鼠

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Abstract:

【】 目的 研究肥大细胞膜稳定剂色甘酸二钠(disordiumcromoglycate,DC)对葡聚糖硫酸钠(dextransulfatesodium,DSS)诱导的大鼠急性结肠炎的影响。 方法 出生80~100d的雄性SD清洁级大鼠30只,体重180~250g。30只大鼠随机分为3组正常对照组(A组)、溃疡性结肠炎组(B组)和DC组(C组),每组各10只。B组和C组自由饮用40g/LDSS溶液(4%)7d诱发急性结肠炎,同时C组每天按100mg/kg腹腔注射DC1次,A组和B组每天腹腔注射等量的生理盐水。7d后,处死各组大鼠。对各组大鼠行疾病活动指数评分,结肠组织行大体评分、组织学评分,检测门静脉血一氧化氮浓度,结肠组织髓过氧化物酶活性。 结果 疾病活动指数评分、大体评分、组织学评分、一氧化氮浓度及髓过氧化物酶活性均表现为B组>C组>A组(P<0.05)。 结论 肥大细胞膜稳定剂DC对DSS诱导的大鼠急性结肠炎有一定的保护作用。【Abstract】 Objective Toobservetheinfluenceofthemastcellmemebranestabilizer,disordiumcromoglycate(DC),ondextransulfatesodium(DSS)-inducedcolitisinrats. Methods ThirtymaleSprague-Dawley(SD)ratsaged80to100dayswiththeirweightrangedfrom180to250gwererandomlydividedinto3groupsnormalcontrolgroup(groupA),dextransulfatesodiumgroup(groupB)anddisordiumcromoglycategroup(groupC),with10ratsineach.RatsingroupBandCdrank40g/LDSSsolution(4%)for7daystoinduceacutecolitis.Atthesametime,intraperitonealadministrationofDC(100mg/kg)toratsingroupCwascarriedoutonceaday,whiletheothertwogroupsofratsweregiventhesameamountofnormalsalinesolution.Diseaseactivityindex(DAI),grossandhistologicalevaluationwereanalyzed.NOconcentrationofbloodfromportalveinwasmeasured.Myeloperoxidase(MPO)activityofcolonictissuewasdetected. Results TheexperimentaldataofgroupC,includingDAI,grossevaluation,histologicalassessment,NOconcentrationandMPOactivity,wereallsignificantlyhigherthanthoseofgroupA(P<0.05),butlowerthanthoseofgroupB(P<0.05). Conclusion DisordiumcromoglycatecanprotectthecolonofratswithDSS-inducedacutecolitis.

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