参附注射液对大鼠深Ⅱ度烧伤创面愈合的影响

【】　目的　探讨中成药参附注射液对大鼠深Ⅱ度烧伤创面愈合的影响。　方法　选用16只Sprague-Dawley（SD）大鼠，随机分为实验组、对照组，每组各8只，建立20%体表面积深Ⅱ度烫伤模型。烫伤后即刻及此后每天，实验组大鼠以参附注射液20mL/kg腹腔注射给药，1次/d，连续给药5d；对照组给予注射等剂量的生理盐水。分别于致伤后的7、14d取创面组织块，光学显微镜观察成纤维细胞及胶原纤维生长情况，电子显微镜观察致伤后14d成纤维细胞细胞器情况，分别计算烧伤后第7、14天两组的创面愈合率，并观察比较两组创面愈合的时间。　结果　所有选入实验的SD大鼠均存活至实验结束。实验组、对照组术后第7天创面愈合率分别为（36.34±2.55）%及（33.13±2.62）%，两组差异有统计学意义（t=2.486，P=0.027）；实验组、对照组术后第14天创面愈合率分别为（75.71±2.29）%及（72.36±2.85）%，两组差异有统计学意义（t=2.590，P=0.022）；实验组、对照组创面愈合时间分别为（20.88±1.36）、（22.94±2.16）d，两组差异有统计学意义（t=-2.286，P=0.0395）。伤后7、14d创面组织块切片光学显微镜观察发现，实验组肉芽组织及胶原纤维较多，排列更有序，表皮基底细胞增殖活跃，炎症反应较轻；伤后14d时电子显微镜观察发现，实验组成纤维细胞的细胞器更丰富，分泌胶原更多，实验组创面愈合情况优于对照组。　结论　腹腔注射参附注射液可以促进烧伤创面的愈合，其可能的机制为清除氧自由基，抗脂质过氧化。【Abstract】　Objective　ToexploretheeffectofShenfuinjectiononpromotinghealingofdeeppartial-thicknessburnwoundinrats.　Methods　SixteenSprague-Dawley(SD)ratswererandomlyselected,anddeeppartial-thicknessburnwith20%ofthebodysurfacewasinflicted.Theratswererandomlydividedintoexperimentalgroupandcontrolgroupwitheightineachgroup.RatsintheexperimentalgroupweretreatedwithabdominalinjectionofShenfuinjectionatadoseof20mL/(kg?d)forfivedayscontinually,andratsinthecontrolgroupweretreatedwiththesamedoseof0.9%salinesolution.Thegrowthofgranulationtissueandcollagenfiberswereevaluatedunderlightmicroscopeattheseventhandthefourteenthday.Thegrowthoffibroblastwasobservedundertransmissionelectronmicroscopeatthefourteenthday.Thecurerateofbothgroupsofratsattheseventhandfourteenthdaywascalculated.Thenwecomparedtheirhealingtimeandthecurerateofthetraumaticwoundrespectively.　Results　Allratshadsurviveduntilwoundhealing.Thecurerateattheseventhdayfortheexperimentalgroupandthecontrolgroupwas(36.34±2.55)%and(33.13±2.62)%,andtheirdifferencewasstatistical(t=2.486,P=0.027);Atthefourteenthday,thecureratewasrespectively(75.71±2.29)%and(72.36±2.85)%withasignificantdifferencebetweeneachother(t=2.590,P=0.022).Thehealingtimeoftheexperimentalgroup(20.88±1.36)wassignificantlyshorterthanthatofthecontrolgroup(22.9±2.16)(t=-2.286,P=0.040).Attheseventhandfourteenthday,lightmicroscopeobservationshowedthatthegrowthofgranulationtissueandcollagenfibersforratsintheexperimentalgroupweremuchmorethanthatinthecontrolgroup,thebasalecellproliferationwasmoreactive,andinflammationwasslighter.Throughtransmissionelectronmicroscope,weobservedmorefibroblastandcollagenintheexperimentalgroup,whichshowedabettercurethanthecontrolgroup.　Conclusion　Shenfuinjectioncansignificantlypromotewoundhealingofdeeppartial-thicknessburn.Itmaypossiblygetthiseffectthroughanti-oxidation.