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华西医学  2011 

慢性缺氧对大鼠岩神经节神经元酸敏感离子通道亚型3和亚型2a表达的影响

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Keywords: 岩神经节,慢性缺氧,酸敏感离子通道,大鼠

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Abstract:

【】 目的 探讨慢性缺氧对大鼠岩神经节神经元酸敏感离子通道(acid-sensingionchannels,ASICs)亚型3(ASIC3)和亚型2a(ASIC2a)表达的影响。 方法 将12只健康成年SD大鼠随机分为正常组和缺氧组。用免疫组织化学法(PV)观察正常和慢性缺氧大鼠岩神经节神经元ASIC3和ASIC2a的表达。 结果 给予慢性缺氧刺激后,岩神经节ASIC3阳性表达神经元数目增多(P0.05)。 结论 慢性缺氧可上调大鼠岩神经节神经元ASIC3的表达,而对ASIC2a的表达无明显影响,提示ASIC3和ASIC2a可能在岩神经节对缺氧的反应中起着不同的作用。?【Abstract】 Objective Toinvestigatetheeffectsofchronichypoxiaonexpressionofacid-sensingionchannels(ASIC)3andASIC2ainneuronsofpetrosalganglionsofrats. Methods Atotalof12SDratswererandomlyassignedtocontrolgroupandhypoxiagroup.TheexpressionsofASIC3andASIC2aoftheneuronsinthepetrosalganglionsinthetwogroupswereinvestigatedwiththeimmunohistochemicaltechnique. Results ThelevelofpositiveASIC3expressioninthepetrosalganglionswashigherinthehypoxiagroupthanthatinthecontrolgroup(P0.05). Conclusion ChronichypoxiacansignificantlyincreasetheexpressionofASIC3,butnotthatofASIC2a,oftheneuronsinthepetrosalganglions,suggestingtheirdifferentrolesinmediatingacellularresponsetochronichypoxia.?

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