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华西医学  2011 

腹内高压对肝硬化小鼠肺水通道蛋白1及水通道蛋白5表达的影响

, PP. 165-169

Keywords: 腹内高压,肝硬化,水通道蛋白,肺水肿,小鼠

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Abstract:

【】 目的 探讨腹水引起的腹内高压对肝硬化小鼠肺组织水通道蛋白1(AQP1)和水通道蛋白5(AQP5)表达的影响。 方法 雄性美国癌症研究所(InstitudoofCancerReseach,ICR)小鼠50只,随机取10只作正常对照组(腹压0cmH2O,1cmH2O=0.098kPa),其余?40只用四氯化碳建立肝硬化小鼠模型,并随机分为4组肝硬化(腹压0cmH2O)组、肝硬化(腹压5cmH2O)组、肝硬化(腹压10cmH2O)组、肝硬化(腹压20cmH2O)组,通过腹腔注射不同量的白蛋白生理盐水形成不同的腹压,并维持腹压24h后取肺组织行病理、免疫组织化学、肺湿/干比值及实时荧光定量PCR检测AQP1和AQP5mRNA表达量。 结果 与正常对照小鼠相比,肝硬化小鼠肺AQP5、AQP1表达明显下降(P<0.05);肝硬化小鼠随着腹内压的升高,肺湿/干比值升高,AQP5、AQP1表达相应增加(P<0.05)。 结论 肝硬化可以影响肺AQP1、AQP5的表达;肝硬化小鼠随着腹内压的升高,AQP1、AQP5表达相应增加,并与肺水肿的严重程度密切相关。?【Abstract】 Objective Toinvestigatetheroleofintra-abdominalhypertensioncausedbyascitesontheexpressionofAquaporin(AQP)1andAQP5inthelungofcirrhoticmice. Methods Werandomlychose10from50maleInstitudeofCancerResearch(ICR)micetoformthecontrolgroupintra-abdominalpressure(IAP)=0cmH2O,1cmH2O=0.098kPa.Themodelofcirrhosiswerepreparedbysubcutaneousinjectionofcarbontetrachloridefortherest40micewhichwerethenrandomlydividedinto4groupscirrhosis(IAP=0cmH2O)group,cirrhosis(IAP=5cmH2O)group,cirrhosis(IAP=10cmH2O)group,andcirrhosis(IAP=20cmH2O)group.SalinewithdifferentvolumeofalbuminwasinjectedintotheperitoneumofeachmouseinordertoformdifferentIAP.After24hours,analysisofpathology,immunochemistryandwet/dryratiowasdoneforthelungsofthesemice;andtheexpressionofAQP1andAQP5attheproteinandmRNAlevelswereanalyzedbyIHCandqRT-PCR. Results Comparedwiththenormalmice,theexpressionofAQP1andAQP5inlungsofcirrhoticmiceweresignificantlylower(P<0.05).Boththelungwet/dryratioandtheexpressionofAQP1andAQP5raisedwiththeincreaseofIAP. Conclusion CirrhosiscanaffecttheexpressionofAQP1andAQP5inlungs.TheexpressionofAQP5andAQP1inlungsofcirrhoticmiceincreaseswiththeincreaseofIAP,whichisalsocloselycorrelatedwiththeseverityofpulmonaryedema.

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