抗氧化应激参与参附注射液预处理诱导的肾脏保护作用

【】　目的　探讨抗氧化应激是否参与参附注射液预处理诱导的肾脏保护作用。　方法　健康成年雄性SD大鼠21只随机分为假手术对照组（Sham组）、肾脏缺血再灌注组（I/R组）和参附注射液组（SF组）；SF组给予参附注射液10mL/kg腹腔注射，每日1次，连续给药7d。麻醉下行右肾切除后，用无损伤动脉夹钳夹左侧肾蒂60min，再灌注24h，制备肾缺血再灌注损伤动物模型。比较各组SD大鼠再灌注24h肾脏组织中超氧化物歧化酶（superonidedismutase，SOD）水平、过氧化氢酶（catalese，CAT）和丙二醛（malonicalaldehyed，MDA）含量。　结果　与Sham组相比，I/R和SF组肾脏组织SOD和CAT显著降低，而MDA明显升高（P<0.05）；与I/R组比，参附注射液能明显增加SOD和CAT水平（P<0.05），降低MDA含量（P<0.05）。　结论　参附注射液预处理可增强缺血再灌注损伤肾脏组织抗氧化应激，其表现为增强SOD和CAT的活力，减少MDA的生成。？【Abstract】　Objective　ToexploretheprotectiveeffectofShenfuinjectioncombinedwithantioxidantsystemonrats’kidneyafterischemia-reperfusioninjury.　Methods　Twenty-onemaleSpragueDawley(SD)ratswererandomlydividedinto3groupsshamoperationgroup(Shamgroup),ischemia-reperfusiongroup(IRgroup),andshenfuinjectiontreatedgroup(SFgroup).Theratswereanesthetizedwithvalebarbitone.Bilateralkidneyswereexposedthroughmidlineincision.Therightkidneyunderwentthenephrectomyandleftrenalpedicelswereoccludedfor60minuteswithatraumaticmini-clampandthenunclampedfor24hours.AnimalsinSFgroupreceivedShenfuinjection(10mL/kg)throughintraperitonealinjectioneverydayfor7days.About24hoursafterreperfusion,superoxidedismutase(SOD),CATandmalonicalaldehyde(MDA)weremeasured.　Results　ThelevelsofMDAwerelowerinSFgroupthanthoseinIRgroup(P<0.05).ThelevelofSODandCATinSFgroupincreasedmoresignificantlythanwhichdidinIRgroup(P<0.05).　Conclusion　OurfindingsuggeststhatantioxidantsysteminSFgroupworksmoreefficientlythanIRgrouptoovercomeoxidativestressinrenalischemia-reperfusioninjury.