[ ２ ] Rabe KF, Hurd S, An zueto A, et al. Global strategy for the diagnosis, management and prevention of chronic obstructive pulmonary disease: GOLD executive summary[J]. Am J Respir Crit Care Med, 2007, 9(6): 532-555．
[3]
[ ３ ] Anzueto AR. Answers to 5 common questions about acute exacerbations of COPD[J]. Postgrad Med, 2009, 121(5): 159-165.
[4]
[ ４ ] Casaburi R, Mahler DA, Jones PW, et al. A long-term evaluation of once -daily inhaled tiotropium in chronic obstructive pulmonary disease[J] . Eur Respir J, 2002, 19(2): 217-224.
[5]
[ 5 ] Vincken W, van Noord JA, Greefhorst APM, et al. Improved health outcomes in patients with COPD during 1 yr’s treatment with tiotropium[J] . Eur Respir J, 2002, 19(2): 209-216.
[6]
[ 6 ] Pauwels RA, Buist AS, Calverley PM, et al. Global strategy for the diagnosis, management, and prevention of chronic obstructive pulmonary disease. NHLBI/WHO Global Initiative for Chronic Obstructive Lung Disease (GOLD) Workshop summary[J]. Am J Respir Crit Care Med, 2001, 163(5): 1256？1276.
[7]
[ 7 ] Kirkpatrick CJ, Bittinger F, Nozadze K, et al． Expression and function of the non-neuronal cholinergic system in endothelial cells [J]. Life Sci, 2003, 72(18/19): 2111-2116．
[8]
[ 8 ] Barnes PJ. Distribution of receptor targets in the lung[J]. Proceed Am Thorac Soc, 2004, 1(4): 345-351．
[9]
[ 9 ] Barnes PJ. Mechanisms in COPD: differences from asthma[J]. Chest, 2000, 117(Suppl 2):10-14.
Calverly PMA, Towse LJ, Lee A. The timing of dose and pattern of bronchodilation of tiotropium (TIO) in stable COPD[J]. Eur Respir J, 2000, 16(31) : 56．
[12]
Disse B, Speck G A, Rominger K L, et al. Tiotropium (spiriva) : mechanical considerations and clinical profile in obstructive lung disease[J]. Life Sci, 1999, 64(6/7): 457-464．
Vacca G, Randerath WJ, Gillissen A. Inhibition of granulocyte migration by tiotropium bromide[J]. Respir Res, 2011, 12(1): 24.
[15]
Koarai A, Traves SL, Fenwick PS, et al. Expression of muscarinic receptors by human macrophages[J]. Eur Respir J, 2012, 39(3): 698-704.
[16]
Pieper MP, Chaudhary NI, Park JE. Acetylcholine-induced proliferation of fibroblasts and myofibroblasts in vitro is inhibited by tiotropium bromide[J]. Life Sci, 2007, 80(24-25): 2270-2273.
