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华西医学  2012 

盐酸氨基葡萄糖在兔关节腔积血中对关节软骨保护作用的实验研究

, PP. 1788-1791

Keywords: 氨基葡萄糖,关节腔积血,软骨细胞,

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Abstract:

目的 观察盐酸氨基葡萄糖对兔关节腔积血造成的软骨损伤的保护作用。方法 成年新西兰大白兔50只,随机分为A、B、C、D、E共5组,每组10只。A、B、C、D组采用自体静脉血4mL右侧关节腔内注射建立关节腔积血模型。建模后,A、B、C组分别给予25、82.5、500mg/kg的盐酸氨基葡萄糖灌胃,1次/d;D组为阳性对照组,以6mL/kg蒸馏水灌胃,1次/d;E组为阴性对照组,以6mL/kg蒸馏水灌胃,1次/d。灌胃8周后取材。肉眼大体观察,光镜下进行Mankin评分及沙红O染色观察,评估软骨损伤程度;采用免疫组织化学法测定Ⅱ型胶原蛋白(COLⅡ)表达情况。结果 软骨大体观察和沙红O染色组织学观察显示,A、B、C组与D组、E组之间差异均有统计学意义(P<0.05)。免疫组织化学提示,A、B、C组COLⅡ表达明显高于D组(P<0.05)。结论 盐酸氨基葡萄糖对兔关节腔积血导致的软骨损伤有一定的保护治疗作用,在防治关节腔积血导致的软骨损伤的发生发展方面,具有一定的作用。

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