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华西医学  2012 

绿茶提取物表没食子儿茶素没食子酸酯对人肝癌细胞株HepG2裸鼠移植瘤新生血管生成的影响

, PP. 877-881

Keywords: 表没食子儿茶素没食子酸酯,原发性肝癌,移植瘤,缺氧诱导因子1α,血管内皮生长因子,微血管密度,新生血管生成

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Abstract:

目的 通过复制人肝癌细胞株HepG2裸鼠皮下移植瘤模型,观察绿茶提取物表没食子儿茶素没食子酸酯(EGCG)干预对HepG2移植瘤新生血管生成的影响。方法 瘤体接种复制HepG2移植瘤模型,荷瘤裸鼠20只随机分组,实验组给予EGCG溶液每日20mg/(kg?只),腹腔注射3周,对照组给予等量灭菌注射用水3周,末次用药24h,后处死裸鼠,剥离移植瘤。常规病理切片观察移植瘤组织结构;逆转录-聚合酶链式反应和免疫组织化学法检测移植瘤缺氧诱导因子-1α(HIF-1α)、血管内皮生长因子(VEGF)mRNA及蛋白表达,并通过检测CD34表达计数瘤组织微血管密度(MVD)。结果 组织病理学观察实验组移植瘤见大量坏死区,瘤体内血管数量明显少于对照组;实验组HIF-1α、VEGFmRNA及蛋白表达水平比对照组均明显下调(P<0.05),实验组MVD比对照组明显下降(P<0.05)。结论 EGCG可抑制荷瘤裸鼠HepG2移植瘤新生血管生成。

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