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华西医学  2012 

利多卡因惊厥对大鼠空间学习能力的影响

, PP. 545-548

Keywords: 利多卡因,惊厥,海马,神经毒性,空间学习能力,大鼠

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Abstract:

目的 研究利多卡因对海马的神经毒性是否会对大鼠空间学习记忆能力产生影响,并探讨大鼠空间学习能力的变化与海马CA3区锥体细胞数目的相关性。方法 将成年Wistar雄性大鼠随机分为基础值组(n=7)和利多卡因惊厥组(n=40)。基础值组大鼠静脉给予生理盐水后使用Y迷宫测定大鼠的空间学习能力。利多卡因惊厥组大鼠尾静脉持续输注利多卡因造成惊厥,待大鼠恢复正常运动以后放入鼠笼重新饲养。并于惊厥后第1、3、5、7天从中随机抓取大鼠测试其空间学习能力以及组织学改变。根据对应天数将利多卡因惊厥组的40只大鼠随机细分为Day-1、Day-3、Day-5、Day-7亚组,每亚组10只。所有大鼠在测定空间学习能力之后立即处死,取出大脑并做石蜡包埋,冠状面切片后进行组织学检测,显微镜下评估海马CA3区锥体细胞状态。结果 ①基础值组和Day-1、Day-3、Day-5、Day-7亚组大鼠的Y迷宫穿梭次数分别为(25.2±3.7)、(27.1±8.1)、(36.9±9.9)、(38.7±10.6)、(40.6±16.3)次,除Day-1亚组与基础值组比较差异无统计学意义(P>0.05)外,其余各亚组与基础值组差异均有统计学意义(P<0.05);②与基础值组单位面积(10.3±4.5)个(异常锥体)细胞比较,利多卡因惊厥组大鼠海马CA3区异常锥体细胞数增加,Day-1、Day-3、Day-5、Day-7亚组计数值分别为13.0±7.2、15.6±5.0、19.6±8.1、18.1±5.1,且与大鼠Y迷宫穿梭次数呈正相关(r=0.711,P<0.05)。结论 利多卡因引起的惊厥使成年大鼠海马依赖性空间学习能力下降,利多卡因的神经毒性引起的海马异常锥体细胞增多可能是造成这一现象的一种原因。

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