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杜仲基因组微卫星特征及SSR标记开发

Keywords: 杜仲 基因组 SSR 标记开发 多态性

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Abstract:

为全面解读杜仲的遗传背景, 本研究在基因组测序(数据未公布)的基础上, 通过MISA软件搜索杜仲基因组(26 947/854 758 160 bp)中的完整型(17核苷酸重复)及复合型SSR序列, 共查找出25 694个Scaffolds含有488 592个SSR位点, 占总Scaffolds的95.3%。从SSR位点分布密度上讲, 平均每1 749 bp出现1个微卫星, 其中单核苷酸重复单元的SSR含量最多, 约占总数的54.34%, 其次为二核苷酸 (20.47%), 而复合模式、三核苷酸、四核苷酸、五核苷酸、六核苷酸和七核苷酸重复分别占20.29%、23.89%、0.77%、0.13%、0.10%和0.01%, 并发现SSR中均以含A、T的重复类型占主导地位。根据SSR位点设计并合成引物290对, 162对SSR引物扩增出清晰的目的条带, 其中16对引物多态性高、稳定性好, 在8份杜仲资源中共检测到84个等位基因, 平均每个SSR位点检测到5.25个等位基因。本研究对于进一步利用SSR分子标记分析杜仲遗传多样性、遗传图谱构建、杜仲雌雄株早期鉴定等具有较高的应用价值

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