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孝顺竹RNA提取方法研究

, PP. 769-772

Keywords: 孝顺竹,RNA,提取,改良SDS法

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Abstract:

提取纯度高、完整性好、不含DNA或其它杂质 的总RNA是进行Northern杂交、cDNA合成、cDNA 文库构建、RT-PCR及mRNA差异显示等分子生物 学研究的基础。目前提取植物RNA方法有多种,这 些方法设计旨在RNA提取过程中清除多糖、多酚等 杂质污染。在提取高质量RNA的过程中,植物尤其 木本植物通常含有大量的酚类、多糖和其他一些水 溶性化合物与RNA共沉淀,影响了RNA功能。

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