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甜菜碱合成调控基因共表达载体的构建与抗盐初步研究

, PP. 231-235

Keywords: 甜菜碱,表达载体,抗盐

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Abstract:

甘氨酸甜菜碱是植物体内重要的渗透调节物质,通过逐步克隆方法,将甜菜碱合成途径中编码三个关键酶(PEAMT、CMO和BADH)的基因构建到同一表达载体中,并经过酶切检测,证明得到了正确表达载体质粒---pCBP。将pCBP转入农杆菌GV3101,用真空渗透法转化拟南芥(Col-0)植株,通过抗性筛选获得了转基因植株,PCR检测证实三个目的基因已经转入到拟南芥植株中。抗盐性检测表明,在含NaCl125mmol?L-1的1/2MS培养基上,转基因植株的生长明显优于Col-0。

References

[1]  赵可夫,李法曾.中国盐生植物[M].北京:科学出版社,1999
[2]  Sentenac H, Bonneaud N, Minet M,et al. Cloning and expression in yeast of a plant potassium ion transport system[J]. Science, 1992, 256:663~665
[3]  McNeil S D, Nuccio M L, David R, et al. Radiotracer and computer modeling evidence that phospho-base methylation is the main route of choline synthesis in tobacco[J]. Plant Physiology, 2000, 123:371 ~380
[4]  Huang J, Hirji R, Adam L, et al. Genetic engineering of glycinebetaine production toward enhancing stress tolerance in plants: metabolic limitations[J]. Plant Physiol, 2000, 122:747~756
[5]  McNeil S D, Rhodes D, Russell B L, et al. Metabolic modeling identities key constraints on an engineered glycine betaine synthesis pathway in tobacco[J]. Plant Physiol, 2000, 124(1):153~162
[6]  Sambrook J, Fritsch E F, Maniatis T. Molecular Cloning[M]. Cold Spring Harbor Laboratory Press, 1989
[7]  Mou Z, Wang X, Fu Z, et al. Silencing of phosphoethanolamine N-methyltransferase results in temperature-sensitive male sterility and salt hypersensitivity in Arabidopsis[J]. Plant Cell, 2002, 14(9):2031~2043

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