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树木溃疡病重要病原葡萄座腔菌属、种及其无性型研究

, PP. 21-28

Keywords: 葡萄座腔菌,小穴壳属,壳梭孢属,无性型,同物异名

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Abstract:

本实验在我国的多种树木上采集了56个溃疡病标本,采用形态学方法和分子生物学方法( ISSR技术)研究病原菌。通过徒手切片和石腊切片观察,供试病原菌有性型为B otryosphaeria dothidea;无性型特征与国外所报道的七叶树壳梭孢(Fusicoccum aesculi)一致。根据ISSR技术得到的系统树显示,供试菌株与国外提供的F. aesculi (有性阶段为B. dothidea)菌株以100%相似性聚为一类。因此,实验采集的树木溃疡病菌是B. dothidea,其无性型不是Dothiorella gregaria,而是F. aesculi,故应使用F. aesculi作为B. dothidea的无性型名称。此外,通过国内的B. dothidea和国外已发表的B. ribis的特征对照,发现二者确实不同: B. ribis的分生孢子宽度大于8. 0μm, L /D值的平均值小于2. 6;而本实验测量的B. dothidea的分生孢子宽度在6. 2~7. 6μm之间, L /D值的平均值在2. 9~3. 6之间; ISSR所得系统树也显示二者属于不同的种。所以B. dothidea和B. ribis是两个不同的种,而不是同物异名。

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