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乳牛肝菌属分离株分子鉴定及其遗传关系的研究

, PP. 825-831

Keywords: 外生菌根真菌,乳牛肝菌属,ITS,分子鉴定,遗传多样性,ITS-RFLP,序列分析

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Abstract:

本文采用ITS序列分析方法和ITS-RFLP分子标记方法对内蒙古大青山地区分离获得的27个乳牛肝菌属(Suillus spp. )真菌菌株进行了分子鉴定和遗传关系研究。通过ITS序列比对鉴定出SP1、SP2、SP3、SP4、SP5、SP6、SP7、SP8、SP9、SF1、SF2、SF3、P. Lac-1、P. Lac-2、3′、3″、3#菌株属于褐环乳牛肝菌(Suillus luteus) 。27株乳牛肝菌属真菌分为3个遗传类型,且每个遗传类型在分类上属于同一个种。樟Ⅱ和1"与其余菌株的遗传距离较大,其原因可能是宿主和生境的影响。序列分析方法能够更准确地分析出遗传差异,但ITS-RFLP方法更简单、实用,可以用于初步的菌株鉴定。两种方法结合可以更有效和准确地鉴定外生菌根真菌和研究其遗传关系。

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