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EST-CAPS标记在尾叶桉和细叶桉遗传图谱构建中的应用

, PP. 230-234

Keywords: EST-CAPS,尾叶桉,细叶桉,遗传图谱

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Abstract:

以已构建的尾叶桉和细叶桉RAPD连锁图谱为基础,利用CAPS技术对54个细叶桉EST序列在尾叶桉和细叶桉遗传图谱上的定位研究表明: 7个EST序列在作图群体中呈等位片段多态性,包括母本尾叶桉特有的3个(其中1个偏分离) 、父本细叶桉特有的2个和父母本共有的2个;共有4个EST-CAPS标记整合到尾叶桉RAPD连锁图谱,分散于不同的连锁群,各连锁群大小均有小幅增加;细叶桉RAPD连锁图谱上也整合了4个EST-CAPS标记,分散于不同的连锁群,各连锁群大小均有不同程度的增加;另外,尾叶桉上的1个偏分离标记未整合到任何连锁群。EST-CAPS可以有效用于桉属树种遗传图谱构建。

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