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坡柳种子对脱水干燥的生理生化响应

, PP. 619-626

Keywords: 坡柳,抗氧化酶,脂质过氧化,超干燥保存

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Abstract:

为研究超干燥保存手段运用于坡柳种子的可行性,采用正交设计从种子含水量、包装方法、保存温度和预回湿方法4个因素来分析。用硅胶将种子脱水至1.12%~6.13% 6个含水量梯度,结合不同处理密封保存1年,随后测定发芽率和一些生理生化指标,包括相对电导率(RC)、脯氨酸(Pro)、丙二醛(MDA)、超氧化物歧化酶(SOD)、过氧化物酶(POD)、过氧化氢酶(CAT)、可溶性总糖含量和脂肪酸组分。结果表明:种子含水量是对发芽率影响最显著的因素,较为适宜的脱水范围为4.28%~5.25%;超干种子发芽率与相对电导率和丙二醛含量呈显著负相关,与脯氨酸含量和3种抗氧化酶活性呈显著正相关;超干种子的油酸和α-亚麻酸含量比对照提高。坡柳种子保存过程中的劣变是由细胞膜损伤和脂质过氧化所引起,而适宜的脱水处理能使抗氧化酶活性增强,使不饱和脂肪酸的含量增加,从而维持种子生活力的稳定。

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