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一种新的白蜡虫寄生蜂体内Wolbachia的wsp基因分子检测及序列分析

, PP. 340-344

Keywords: 白蜡虫,长尾啮小蜂,Wolbachia,wsp

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Abstract:

采用Wolbachia的通用引物、A大组和B大组的特异性引物对一种新的白蜡虫寄生蜂――长尾啮小蜂(Aprostocetus sp.)体内Wolbachia的wsp基因进行分子检测,所获得的基因片段分别命名为wApr、wAprA和wAprB,长度分别为620、566和463 bp;基因序列分析表明:wAprA、wAprB与wApr在对应位置序列仅有4个碱基的差异。系统发育分析表明:该寄生蜂仅感染了B大组Dig亚组的Wolbachia。wApr和wAprB基因序列已经递交到NCBI,登录号分别是HQ121415和HQ121417。

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