全部 标题 作者
关键词 摘要

OALib Journal期刊
ISSN: 2333-9721
费用:99美元

查看量下载量

相关文章

更多...

灰树花的系统发育分析和主要木质素降解酶的测定

, PP. 574-580

Keywords: 灰树花,ITS序列,木质素降解酶系统,锰过氧化物酶,漆酶

Full-Text   Cite this paper   Add to My Lib

Abstract:

对灰树花菌株迁西二号进行了ITS序列扩增与测序(GenBank登录号为GU584099),并对灰树花及相关白腐菌进行了基于ITS序列的系统发育分析,结果表明灰树花与Grifola sordulenta (Mont.) Singer等白腐菌的亲缘关系较近。采用LNAS(低氮天冬酰胺-琥珀酸)培养基添加4种不同底物,对菌株迁西二号在25 ℃恒温下静置培养,得到了不同时间内的培养液,用紫外可见分光光度计分别检测了在470 、420 、310 nm处对于2,6-二甲氧基苯酚 (2,6-DMP)、2,2’-连氮-双(3-乙基苯并噻唑-6-磺酸)(ABTS)、3,4-二甲氧基苯甲醇/藜芦醇(VA)的氧化作用后光密度值的变化情况,作为锰过氧化物酶(MnP)、漆酶(Laccase)和木质素过氧化物酶(LiP)产生和活性大小的依据,从而获得了灰树花木质素降解酶系统的主要酶系及其与培养基的成分和酶作用底物的基本关系。结果表明,灰树花可产生MnP和漆酶,但不产生LiP;对比4种成分不同的培养液酶活力测定结果,未添加底物的培养液MnP最大酶活仅为2.96 U?L-1,漆酶最大酶活仅为4.49 U?L-1。添加底物木屑和2,6-DMP后MnP最大酶活为8.06 U?L-1,漆酶最大酶活为9.85 U?L-1,表明在培养液内添加酶的底物木屑后能轻微地提高两种酶的分泌量。

References

[1]  张美彦, 尚晓冬, 李 玉, 等. ITS2RFLP及SRAP标记在灰树花种质评价中的应用[J] . 食用菌学报, 2009,16 (3) : 5-10
[2]  Maeda Y, Kajiwara S, Ohtaguchi K. Manganese peroxidase gene of the perennial mushroom Elfvingia applanata:cloning and evaluation of its relationship with lignin degradation[J] . Biotechnology Letters, 2001, 23: 103-109
[3]  Lankinen P, Hilden K, Aro N, et al. Manganese peroxidase of Agaricus bisporus: grain bran-promoted production and gene characterization[J]. Appl Microbiol Biotechnol, 2005, 66:401-407
[4]  Alic M, Akileswaran L, Gold M H. Characterization of the gene encoding manganese peroxidase isozyme 3 from Phanerochaete chrysosporium[J]. Biochimica et Biophysica Acta, 1997, 1338:1-7
[5]  Tello M, Corsini G, Larrondo L F, et al. Characterization of three new manganese peroxidase genes from the ligninolytic basidiomycete Ceriporiopsis subvermispora[J]. Biochimica et Biophysica Acta, 2000, 1490:137-144
[6]  Irie T, Honda Y, Ha H C, et al. Isolation of cDNA and genomic fragments encoding the major manganese peroxidase isozyme from the white rot basidiomycete Pleurotus ostreatus[J]. J Wood Sci, 2000, 46:230-233
[7]  Johansson T, Nyman P O, Cullen D. Differential regulation of mnp2, a new manganese peroxidase encoding gene from the ligninolytic fungus Trametes versicolor PRL 572[J]. Applied and Environmental Microbiology, 2002, 68:2077-2080
[8]  Hildén K, Martinez A T, Hatakka A, et al. The two manganese peroxidases Pr-MnP2 and Pr-MnP3 of Phlebia radiata, a lignin-degrading basidiomycete, are phylogenetically and structurally divergent[J]. Fungal Genetics and Biology, 2005,42:403-419
[9]  孙 迅. 粗毛栓菌的木质纤维素降解酶及其基因克隆 . 成都:四川大学, 2004
[10]  官 敏. 好食脉孢菌产锰过氧化物酶培养条件的优化、酶的纯化以及酶学性质的分析 . 广州:暨南大学, 2006
[11]  闫洪波. 偏肿拟栓菌锰过氧化物酶cDNA基因克隆及在毕赤酵母中的表达 . 哈尔滨:东北林业大学, 2009
[12]  张银波, 姜 琼, 江木兰, 等. 金针菇漆酶基因的克隆及其在毕赤酵母中的表达研究[J] . 微生物学报,2004 , 44(6):775-779
[13]  谌 斌, 唐雪明, 沈 微. 粗糙脉孢菌漆酶基因的克隆及在毕赤酵母中的初步表达[J] .食品与生物技术学报, 2006, 25(4):43-54
[14]  Cui F J, Tao W Y, Xu Z H, et al. Structural analysis of anti-tumor heteropolysaccharide GFPS1b from the cultured mycelia of Grifola frondosa GF9801 [J]. Bioresource Technology, 2007, 98:395-401
[15]  Lee B C, Bae J T, Pyo H B, et al. Biological activities of the polysaccharides produced from submerged culture of the edible Basidiomycete Grifola frondosa [J]. Enzyme and Microbial Technology, 2003, 32: 574?581
[16]  王海燕. 灰树花GPD启动子及药效相关基因的克隆 . 海口: 华南热带农业大学, 2004
[17]  崔凤杰. 灰树花深层发酵条件优化及其菌丝体抗肿瘤糖肽的研究 . 无锡: 江南大学, 2006
[18]  池玉杰, 李俊涛, 刘智会. 灰树花的培养特性与液体菌种栽培技术[J]. 东北林业大学学报, 2007, 35(3) : 49-52, 58
[19]  Shen Q, Geiser D M, Royse D J . Molecular phylogenetic analysis of Grifola frondosa (maitake) reveals a species partition separating eastern North American and Asian isolates [J]. Mycologia, 2002, 94(3) : 472?482
[20]  Nagai M, Sakamoto Y, Nakade K, et al. Isolation and characterization of the gene encoding a manganese peroxidase from Lentinula edodes[J]. Mycoscience, 2007, 48: 125-130
[21]  Kirk T K, Schultz E, Conors W J, et al. Influence of culture parameters on lignin melabolism by Phanerochaete chrysosporium[J]. Arch Microbiol, 1978, 117: 277-285
[22]  Hatakka A, Uusi-r A K. Degradation of 14C-labelled poplar wood lignin by selected white-rot fungi[J]. Eur J Appl Microbiol Biotechnol, 198 , 17:235-242
[23]  Eggert C, Temp U, Eriksson K E. The ligninolytic system of the white-rot fungus Pycnoporus cinnabarinus: purification and characterization of the laccase [J]. Applied and Environmental Microbiology, 1996, 62 : 1151-1158
[24]  WariishiI H, Valli K, Gold M H. Manganese (II) oxidation by manganese peroxidase from the basidiomycete Phanerochaete chrysosporium kinetic mechanism and role of chelators[J]. Journal of Biological Chemistry, 1992, 267(33) : 23688-23695
[25]  Tien M, Kirk T K. Lignin peroxidase of Phanerochaete chrysosporium[J]. Methods in Enzymology, 1988, 161: 238-249
[26]  Chi Y J, Hatakka A, Maijala P. Can co-culturing of two white-rot fungi increase lignin degradation and the production of lignin-degrading enzymes?[J]. International Biodeterioration and Biodegradation, 2007, 59(1): 32-39
[27]  池玉杰, 闫洪波. 红平菇木质素降解酶系统漆酶、锰过氧化物酶及木质素过氧化物酶的检测[J] . 林业科学, 2009, 45(12):154-158

Full-Text

Contact Us

service@oalib.com

QQ:3279437679

WhatsApp +8615387084133