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TNF-α基因启动子区-863C/A多态性与慢性乙肝易感相关性

, PP. 447-451

Keywords: 肿瘤坏死因子-α,-863C/A多态性,慢性乙型肝炎,易感性

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Abstract:

目的研究肿瘤坏死因子-α(TNF-α)启动子区-863C/A位点单核苷酸多态性(SNP)与慢性乙型肝炎(CHB)易感相关性。方法采用聚合酶链反应-限制性片段长度多态性(PCR-RFLP)方法对142例慢性乙型肝炎患者(CHB组)和150名健康者(HC组)的TNF-α基因启动子区-863C/A位点单核苷酸多态性进行基因分型,以肝功能指标(ALT、AST、TBIL、GGT、ALB)、乙型肝炎E抗原(HBeAg)、HBV-DNA及其病毒载量、HBV-LP、HBV-PreS1作为检测指标。结果CHB组与HC组比较,TNF-α-863位点CA&AA基因型组间频率分布差异有统计学意义(χ2=4.222,P=0.040),等位基因A与CHB易感相关(OR=1.641,OR95%CI=1.022~2.636);TNF-α-863C/A多态性与ALT、AST、HBeAg、HBV-DNA、HBV-LP、HBV-PreS1阳性率无统计学关联(P>0.05);等位基因A与C比较,HBV-DNA病毒载量在104~105时,频率差异具有统计学意义(χ2=5.558,P=0.018)。结论TNF-α基因启动子-863C/A位点SNP与CHB易感相关,等位基因A可能是HBV的易感基因。

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