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盐酸戊乙奎醚对内毒素血症大鼠紧密连接蛋白表达的影响

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Keywords: 盐酸戊乙奎醚,脂多糖,急性肾损伤,紧密连接蛋白

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Abstract:

目的观察盐酸戊乙奎醚(PHC)对脂多糖(LPS)致大鼠急性肾损伤的作用,并探讨其对内毒素血症大鼠紧密连接闭锁小带蛋白(ZO-1)和紧密连接闭锁蛋白(Occludinprotein)表达的影响。方法成年雄性SD大鼠随机分为对照组(C组)、LPS组(LPS组)、PHC低剂量组(L-PHC)和PHC高剂量组(H-PHC),每组10只,后3组注入LPS(5.0mg/kg),30min后,L-PHC和H-PHC组分别注入PHC0.3、0.9mg/kg,24h后取外周血和肾组织,检测血清肌酐(Scr)、尿素氮(BUN)、TNF-α和IL-1β含量;蛋白免疫印迹法检测ZO-1和occludin蛋白的表达。结果LPS组肾脏炎症明显,而L-PHC和H-PHC组肾脏炎症较弱;与C组比较,各组血浆TNF-α、IL-1β、Scr和BUN含量均显著升高(P<0.05),而L-PHC和H-PHC组其含量比LPC组明显降低(P<0.05);ZO-1和occludin蛋白表达水平LPS组升高,L-PHC和H-PHC组比LPS组下降(P<0.05)。结论PHC可减轻脓毒症诱发的AKI,调控ZO-1和occludin蛋白的表达可能是其发挥保护作用的机制之一。

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