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尼古丁对脐带间充质干细胞微观形貌及增殖活性的影响

DOI: 10.13604/j.cnki.46-1064/r.2015.08.02, PP. 912-916

Keywords: 尼古丁,间充质干细胞,增殖,凋亡,微观形貌

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Abstract:

目的探讨尼古丁对脐带间充质干细胞(MSCs)形态学、细胞周期、细胞增殖及凋亡的影响。方法不同浓度尼古丁作用于MSCs,以相差显微镜及原子力显微镜(AtomicForceMicroscope,AFM)观察形态学变化;MTT法分别于24h、48h、72h检测细胞增殖;流式细胞仪法于24h检测细胞周期与细胞凋亡。结果尼古丁作用于MSCs后,细胞固缩,细胞膜表面形成空洞及凹陷,微绒毛样突起多见;细胞周期改变,G0/G1期的细胞比例明显增加(PPP>0.05);1mg/mL组凋亡率(10.333±0.961)%、1.5mg/mL组凋亡率(44.367±3.612)%与对照组比较,差异均有统计学意义(P结论尼古丁使MSCs形态及超微结构发生早期凋亡改变,阻滞细胞周期,抑制细胞增殖且促进其凋亡。

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